Ali Aghebati-Maleki
1 
, Behzad Baradaran
1, 2 , Khalil Hajiasgharzadeh
1 , Babak Bakhshinejad
3 , leili Aghebati-Maleki
1* 1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Phage display is a method of studying protein-protein, protein-peptide, and protein-DNA that uses bacteriophages to bind proteins with genetic information that encodes them. Phage display is a state-of-the-art technology for the production, identification, and engineering of fully human antibodies. Thus, many antibodies have been delivered to the medical community for research and clinical applications. In addition, next-generation sequencing to change their perspective on the mechanisms of human disease and has blended both clinical and basic research. Recent molecular biology techniques have greatly benefited from the development of this technique. Next-generation sequencing has created a potential and reliable platform for the development of therapeutic monoclonal antibodies, providing an unprecedented insight into library diversity and clonal enrichment. In this study, we will briefly outline the applications of next-generation sequencing in the biological sciences.