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ImmunoAnalysis. 2022;2: 12.
doi: 10.34172/ia.2022.12
  Abstract View: 192
  PDF Download: 199

Original Research

Study of LIF, NR3C1, BCL6, miR-494, and miR-30d in Endometrial Epithelial Cells of Repeated Implantation Failure and Healthy Women Treated with Calcitriol and Letrozole in a Fibrin Scaffold

Sepide Goharitaban 1,2 ORCID logo, Kobra Hamdi 3, Mozafar Khazaei 4, Masoumeh Esmaeilivand 5, Behrooz Niknafs 1,2,6* ORCID logo

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran
3 Womens Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Department of Nursing, School of Nursing & Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran
6 Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Corresponding Author: Behrooz Niknafs, Email: , Email: niknafsbeh@yahoo.com

Abstract

Background: Determining the exact time of window of implantation (WOI) in individuals with repeated implantation failure (RIF) is necessary for successful implantation. The present study aimed to compare the effects of letrozole and/or calcitriol on the expression of receptivity markers in endometrial epithelial cells (EECs) of RIF patients and healthy fertile women.

Methods: Endometrial tissue was obtained from RIF patients (n=5) and healthy women (n=5) in the proliferation phase. EECs were isolated and cultured in a fibrin scaffold in 10 groups. The cells were treated with letrozole and/or calcitriol in three types of treatment. To check the proliferation and migration of cells in the scaffold, immunofluorescent and H&E staining was used, and gene expression was checked using real-time polymerase chain reaction (PCR).

Results: This study showed that the expression levels of pro-implantation genes (NR3C1, LIF, and miR-30d) were higher in healthy women than in RIF patients, and the expression levels of anti-implantation genes (BCL6 and miR-494) were lower in healthy women than in RIF patients. Calcitriol and letrozole increased the expression level of pro-implantation genes (NR3C1, LIF, and miR-30d) in RIF patients. Furthermore, they reduced the expression level of anti-implantation genes (BCL6 and miR-494) in RIF patients.

Conclusion: Our findings suggest that treatment with letrozole is more effective than co-treatment with letrozole and calcitriol or alone treatment with calcitriol on receptivity markers in RIF patients.

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Submitted: 03 Dec 2022
Accepted: 21 Dec 2022
ePublished: 27 Dec 2022
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