Abstract
Background: One of the new emerging techniques to preserve reproductive potential of cancer patients is cryopreservation of ovarian fragments prior to medical treatment and their re-transplantation after healing. In order to investigate and compare apoptosis in ovarian tissue, the current study has done while using different vitrification solutions.
Methods: The ovaries of Balb/c mice were removed from the body. R ovaries autotransplanted and L ovaries vitrified and thawed in different solutions. Apoptosis-related genes (Bax, Bcl-2, Bid, P53) assessed by RT-PCR.
Results: The results have shown that Bax and P53 expression showed significant changes in DCV3, but in DCV2 only p53 expression decreased considerably in vitrified-thawed group.
Conclusion: We concluded that cryoprotectant solution is important for preserving tissue in vitrification process and in our study DCV2 showed better results than DCV3.