Tabriz University of Medical Sciences ImmunoAnalysis 2783-2589 3 1 2023 03 27 Assessment of Apoptosis-Related Genes in Autografted/ Vitrified Ovarian Tissue in Mice 13 13 10.34172/ia.2023.13 EN Melika Izadpanah https://orcid.org/0000-0003-1227-6875 Mohammad Reza Aliparasti Ali Abedelahi https://orcid.org/0000-0002-9489-8178 Journal Article 10.34172/ia.2023.13 2023 06 02 2023 11 12 Background: One of the new emerging techniques to preserve reproductive potential of cancer patients is cryopreservation of ovarian fragments prior to medical treatment and their re-transplantation after healing. In order to investigate and compare apoptosis in ovarian tissue, the current study has done while using different vitrification solutions. Methods: The ovaries of Balb/c mice were removed from the body. R ovaries autotransplanted and L ovaries vitrified and thawed in different solutions. Apoptosis-related genes (Bax, Bcl-2, Bid, P53) assessed by RT-PCR. Results: The results have shown that Bax and P53 expression showed significant changes in DCV3, but in DCV2 only p53 expression decreased considerably in vitrified-thawed group. Conclusion: We concluded that cryoprotectant solution is important for preserving tissue in vitrification process and in our study DCV2 showed better results than DCV3.